See the Oxygen molecules bubble and the indicator turn pink

Hydrolysis Water Splitting
Using a 9V battery, 2 electrodes and small gauge wire, you can split water into its component parts. This process is called hydrolysis. We add a small amount of salt to increase the conductivity of the water and an acid/base indicator to visualize the reaction.

The chemical formula of water is H2O. When the electrical current, produced by the battery, passes through the water,  the water will split and the two electrodes will bubble. Hydrogen will appear at the cathode and the oxygen at the anode. The acid base indicator around the cathode will turn blue (because the free OH molecules raise the pH) and the area around the anode will turn pink (because the free hydrogen molecules lower the pH).

Looking at the formula for water, there are twice as many hydrogen atoms as oxygen. When hydrolysis occurs, twice as many hydrogen bubbles will be released as oxygen. You can visually see extra bubbles at the point where hydrogen is being released.

Hydrolysis experiments can be quantitative (how much hydrogen and oxygen are released?) or qualitative (can I visually see the reaction taking place?)

Praying Mantid Eggcase

What is the difference between complete and incomplete metamorphosis in insects?

Incomplete and complete metamorphosis differ in the number of life cycle stages insects go through during their transformation from egg to adult. Complete metamorphosis has 4 life cycle stages and incomplete metamorphosis has 3 life cycle stages.

Complete Metamorphosis

Complete metamorphosis has four distinct life cycle stages: egg, larva, pupa and adult. Examples of insects that go through complete metamorphosis are butterflies, silkworms, mealworms and ladybugs. The larva can be worm-like, although you can still see the six legs. The larvae for moths and butterflies are called caterpillars. Maggots are the larval stage of flies. The larvae eat constantly and grow rapidly. A hard, protective case forms around the larva…this is the pupa stage. The pupa stage for a butterfly is called a chrysallis. The pupa stage for a moth is called a cocoon.

Incomplete Metamorphosis

Incomplete metamorphosis only has three life cycle stages: egg, nymph, adult. The nymph looks similar to, but a smaller version of, the adult. The nymph is also wingless. Examples of insects that go through incomplete metamorphosis are stinkbugs, earwigs, crickets, grasshoppers, cockroaches, ants and praying mantids.

Gram Staining Bacteria Procedure
1.Place a drop of distilled water on a slide and, using a swab or inoculating loop, mix the bacteria with the water an smear the mixture on the slide. The mixture will appear cloudy. Using a flame, heat fix the bacteria to the slide (pass the slide through the flame a few times to “dry” the bacteria and affix it to the slide).

2. Using a dropper, add crystal violet to the slide. Let stand for 1 minute.

3. Add iodine to the slide. Let stand for 3 minutes.

4. Decolorize the sample with alcohol. Let stand for 30 seconds.

5. Counter stain the sample with safranin. Let stand 1-2 minutes. Using a dropper, rinse with distilled water.
Gram Staining Results
Gram positive bacteria will appear purple under the microscope. They have a single, thick cell wall. The crystal violet and iodine combine to attach to this wall. The decolorizer (alcohol) dehydrates the cell wall, causing the pores to close, trapping the stain inside. the safranin added in the final step, does not penetrate the wall.

Gram negative bacteria will appear red. The have a cell wall and additional thin layers of fatty sugars. The decolorizer easily penetrates these thin sugar layers, washing away the crystal violet – iodine chemical (purple color). The safranin in the last step attaches to these layers and appears red.