Archive for June, 2009

owlDissecting owl pellets is a fun and educational method of analyzing predator / prey relationships and for learning basic dissection techniques.

What is an Owl Pellet?

An owl pellet is the portion of an owl’s prey that has not been digested. Owl’s swallow their prey whole (they don’t have teeth to chew) and the feather’s, fur, bones and other undigestible parts are regurgitated by the owl.

How Does the Owl Pellet Form?

When the prey is swallowed, it travels through the esophagus and into the first part of the stomach, the proventriculus. Unlike other birds, the owl does not have a crop to store the food. As a result, the prey enters directly into the digestive tract. This part of the stomach has enzymes and acids (like our stomachs) to aid in digestion. From the proventriculus, the food travels to the second part of the stomach, the gizzard. The gizzard is a muscular organ that grinds the food and ”filters” undigestible parts from traveling into the intestines.

The pellet is formed from the hair, bones or feathers that are left in the gizzard. The pellet will take several hours to form and several more before it is regurgitated. The owl cannot eat again until this pellet is expelled.

Does the Regurgitation of the Pellet Benefit the Owl?

Yes.  Many scientists believe that this regurgitation of the pellet keeps the upper digestive tract clean.

See the Oxygen molecules bubble and the indicator turn pink

See the Oxygen molecules bubble and the indicator turn pink

Hydrolysis Water Splitting
Using a 9V battery, 2 electrodes and small gauge wire, you can split water into its component parts. This process is called hydrolysis. We add a small amount of salt to increase the conductivity of the water and an acid/base indicator to visualize the reaction.

The chemical formula of water is H2O. When the electrical current, produced by the battery, passes through the water,  the water will split and the two electrodes will bubble. Hydrogen will appear at the cathode and the oxygen at the anode. The acid base indicator around the cathode will turn blue (because the free OH molecules raise the pH) and the area around the anode will turn pink (because the free hydrogen molecules lower the pH).

Looking at the formula for water, there are twice as many hydrogen atoms as oxygen. When hydrolysis occurs, twice as many hydrogen bubbles will be released as oxygen. You can visually see extra bubbles at the point where hydrogen is being released.

Hydrolysis experiments can be quantitative (how much hydrogen and oxygen are released?) or qualitative (can I visually see the reaction taking place?)

Praying Mantid Eggcase

Praying Mantid Eggcase

What is the difference between complete and incomplete metamorphosis in insects?

Incomplete and complete metamorphosis differ in the number of life cycle stages insects go through during their transformation from egg to adult. Complete metamorphosis has 4 life cycle stages and incomplete metamorphosis has 3 life cycle stages.

Complete Metamorphosis

Complete metamorphosis has four distinct life cycle stages: egg, larva, pupa and adult. Examples of insects that go through complete metamorphosis are butterflies, silkworms, mealworms and ladybugs. The larva can be worm-like, although you can still see the six legs. The larvae for moths and butterflies are called caterpillars. Maggots are the larval stage of flies. The larvae eat constantly and grow rapidly. A hard, protective case forms around the larva…this is the pupa stage. The pupa stage for a butterfly is called a chrysallis. The pupa stage for a moth is called a cocoon.

Incomplete Metamorphosis

Incomplete metamorphosis only has three life cycle stages: egg, nymph, adult. The nymph looks similar to, but a smaller version of, the adult. The nymph is also wingless. Examples of insects that go through incomplete metamorphosis are stinkbugs, earwigs, crickets, grasshoppers, cockroaches, ants and praying mantids.

Keep the lid over your plate to prevent contamination.

Keep the lid over your plate to prevent contamination.

All living organisms require energy. They can get their energy from multiple sources: organic chemicals(carbon containing compounds), inorganic chemicals and light. Bacteria use organic chemicals, such as, sugars, starch, protiens and fats to grow. Bacteria are called heterotrophs.

Most bacteria grow best at normal, human body temperature (98-99 degrees F). When growing the bacteria, incubate at a temperature as close to this as possible. The bacteria will grow slower at lower temperatures.

Aseptic technique is the process of growing and transferring bacteria without contaminating the culture by touching or breathing on the sample.

Nutrient agar is a general purpose prepared media and grows many types of bacteria and fungi. If you have a specific bacteria culture, you can spread the bacteria on the plate using a sterile swab or innoculating loop. The bacteria will grow and become visible in 24-48 hrs. If you would like to determine the types of bacteria growing on a sink, chair, table or other areas, a sterile swab can be used to rub across the area you would like to test. After the sample is taken, you can transfer the bacteria to the nutrient agar plate by swiping the swab across the surface of the agar plate. After 24-48 hrs, you may find many, different looking colonies growing on the nutrient agar plate. Each type of bacteria look a little different (color, shape, size) when they grow.